Alcohol Dehydrogenase-1 (Adh-1)
ALLELES: Adh-1F (fast), Adh-1S (slow), Adh-1N (null). Other symbols were used by Felder (1975).
INHERITANCE: AdhF and AdhS codominant, both dominant to AdhN.
PHENOTYPE: The phenotype is based on electrophoresis of liver homogenate: [Electrophoretic conditions. Gel: starch, horizontal. Buffer: Continous, tris glycine, pH 8.3. Visualization. Alcohol with NAD-oxidoreductase substrate and tetrazolium stain.] Zymogram. Mobility: Cathodal. Protein structure: Dimer, intact Homozygotes appear as sinvje electromorphs in the case of AdhF and AdhS. Homozygous AdhN gives no activity. Heterozygotes AdhF/AdhS produce three electromorphs, less intense ones in the "fast" and "slow' Positions and a more intense intermediate band, as expected with a dimeric protein. Heterozygotes AdhF/AdhN and adhS/AdhN exhibit single less intense electromorphs at the "fast" or "slow" positions, respectively. AdhN homozygotes have no alcohol dehydrogenase activity by any assay, and there is no cross reactivity with purified protein from AdhF homozygotes. The null allele either produces no product or a product which is impaired to the extent that it will not cross react immunologically. Homozygous AdhN deer mice have significantly longer sleep times following ethanol induced narcosis.
These sequences are referenced in GenBank
LINKAGE: Linkage Group VI. (Dawson et al., 1983)
DNA SEQUENCE: GenBank Accession # L15703 Bases 1 to 1297.
SOURCE: The AdhF and AdhN alleles occurred in the "BW" laboratory stock of P.m. bairdii, and the AdhS allele is fixed in the "PO" laboratory stock of P.p. subgiiseus ("PO" stock). Both stocks were maintained at the University of South Carolina. Pure lines for the alleles were isolated about 1973 and the
genetics analyzed at the University of South Carolina.
REFERENCES: Felder (1975); Burnett and Felder (1978a, 1978b).
COMMENT: Shaw and Koen (1965) recorded this enzyme in Peromyscus as "nothing dehydrogenase".
 | Starch gel showing alcohol
dehydrognase electrophoretic phenotypes in Peromyscus corresponding to genotypes:
(1) AdhF/AdhF (2) AdhS/AdhS (3)
AdhN/AdhN. Photograph courtesy of M.R. Felder. |
 | Restriction fragment length
variation shown on southern blot using a Peromyscus-specific probe for the Adh-1
locus. |
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This page updated 19 July 2005 by peromyscus@stkctr.biol.sc.edu
This page copyright
2005, The Board of Trustees of the University of South Carolina.